2 edition of radiation inactivation of concanavalina. found in the catalog.
radiation inactivation of concanavalina.
Sahib A. Amir Mudher
PhD thesis, Chemistry.
Low doses (30–84 ergs/mm 2, 1 erg = 10 7 J) of ultraviolet radiation (UV) caused severe inhibition of the proliferation of human lymphocytes in st inhibition was produced when resting cells were irradiated immediately prior to stimulation with concanavalin A (Con A); this was true whether activation was measured by the incorporation of labelled leucine, uridine, or thymidine. Concanavalin A, Fluorescein Conjugate Get the sharpest, brightest images. Try new Invitrogen™ SlowFade™ Glass antifade RIMS mountant with refractive index of
Concanavalin A, Canavalia ensiformis, CAS , is a carbohydrate-binding protein that agglutinates various animal cells. Restores the growth pattern of tumor cells to that of normal cells. Restores the growth pattern of tumor cells to that of normal cells. C.A.S.: First crystallized by Sumner and Howell (), Concanavalin A (ConA) has proven to be an interesting and useful lectin. Lectins are proteins that react with specific terminal sugar residues and are useful probes in studying carbohydrates of cell surfaces.
Wastewater treatment by gamma radiation is a promising technology, with the capacity to reduce the impact of chemical and biological pollution of effluents in the environment. The aim of this study was to find out the effect of gamma radiation on the inactivation response of wastewater microorganisms. Wastewater samples were irradiated at a Co facility, at different dose rates and at. It is necessary to kill covid virus either in high temperature or using high voltage radiation therapy of UV-C so that the DNA material can be dry in dry conditions. Both virus inactivation.
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The radiation inactivation of Concanavalin A Author: Mudher, S. ISNI: Awarding Body: University of Salford Current Institution: University of Salford Date of Award: Availability of Full Text: Full text unavailable from EThOS.
Moyers C, Pottmeyer-Gerber C, Gerber M, Buszello H, Dröge W. The activation of cytotoxic T lymphocytes (CTL) in vivo after immunization of normal or cyclophosphamide-treated mice with allogeneic cells was strongly augmented by the administration of mitomycin C-treated or irradiated concanavalin A Cited by: 3.
The use of and problems associated with biological weapons have been of concern to NATO and non-NATO military organizations for many years. Until recently, most of the readily available literature addressed the military issues associated with the possible use of biological weapons on the battlefield, the medical effects of the various agents, and what was known about medical prophylaxis and.
magnetic waves or electrons to foods. Radiation sources are either gamma rays from cobalt, electron beams or X-rays, and the amount ofirradiation absorbed bya food ismeasured in kGy (1 Gy ¼ 1Jkg)1). Commercial application of ionizing radiation (IR) treatment on foods was started at the beginning of the s, but its success has been Cited by: Concanavalin A is a tetrameric protein with four identical 28 kDa subunits.
29 The minimal structure exhibiting high affinity binding of carbohydrates is the dimeric form. The dimer form of ConA is an active stable structure, and the tetramer–dimer transition can be promoted by low pH or succinylation of the protein. 30 For reasons of economy. The book contains eight chapters and opens with a discussion of the nature of viruses and their relation to physics.
Subsequent chapters present studies on the size, shape, and hydration of viruses; the effects of ionizing radiation on viruses, thermal inactivation of viruses, biological effects of ultraviolet light, sonic and osmotic effects on viruses, and virus genetics and multiplication.
Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean, Canavalia is a member of the legume lectin family.
It binds specifically to certain structures found in various sugars, glycoproteins, and glycolipids, mainly internal and nonreducing terminal α-D-mannosyl and α-D-glucosyl groups. Inactivation by gamma irradiation is partly due to the radiation-induced creation of ozone within the air of samples [23,31].
There is some evidence that the indirect mechanisms of inactivation by gamma irradiation, which includes the effects of ozone, is diminished in frozen samples. Since all of the samples we tested were frozen, it was. The inactivation of eleven viruses by a commercial alcoholic hand disinfectant (‘Desderman’) was tested by three different procedures, viz., in vitro in a standardized suspension test, in vivo.
The activation of cytotoxic T lymphocytes (CTL) in vivo after immunization of normal or cyclophosphamide-treated mice with allogeneic cells was strongly augmented by the administration of mitomycin C-treated or irradiated concanavalin A-activated spleen cells (Con A-spl).
This effect of the Con A-spl was abrogated by treatment with Anti-Thy 1 antibody plus complement, and was therefore.
The human Con A suppressor cell was sensitive to treatment with mitomycin C and to exposure to radiation doses exceeding rads. The Con A suppressor cell was shown to reside in the nylon wool-nonadherent, sheep red cell rosette-forming, histamine receptor-bearing population of lymphocytes and to lack surface DRW antigens.
Differential effects of concanavalin a on acetylcholine and potassium-evoked release of catecholamines from cultured chromaffin cells. Neuroscience6 (9), Liver cancer is the predominant cause of cancer mortality in males of Southern China and Taiwan.
The current therapy is not satisfactory, and more effective treatments are needed. In the search for new therapies for liver tumor, we found that Concanavalin A (Con A), a lectin from Jack bean seeds, can have a potent anti-hepatoma effect.
Inbar M, Sachs L. Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells. Proc Natl Acad Sci U S A. Aug; 63 (4)– [PMC free article] Levinson W, Coleman V, Woodson B, Rabson A, Lanier J, Whitcher J, Dawson C.
Inactivation of herpes simplex virus by thiosemicarbazones and certain cations. Canavanine. Canavanine is the δ-oxa analog of l-arginine, and its oxyguanidinium group has a significantly lower pKa value () than l-arginine () The neutral form of canavanine favors the amino (rather than the imino) tautomer When reacted with ADI, canavanine acts essentially as a.
Written by an international panel of scientists, this book focuses on science and technology and offers thorough coverage of the current use of food irradiation. NATO Advanced Research Workshop on Radiation Inactivation of Bioterrorism Agents ( Budapest, Hungary) Radiation inactivation of bioterrorism agents.
Amsterdam ; Washington, D.C.: IOS Press, (OCoLC) Material Type: Conference publication: Document Type: Book: All Authors / Contributors. THERMAL DEATH IN BACTERIA energy radiation can be expected to cause local- ized indiscriminate damage to the cell along its track, either directly or from the formation of ions or free radicals which cause secondary ef- fects (33).
Heat, on the other hand, will be more or less uniformly distributed in the cell and may be expected to selectively damage only the most sensitive molecules within. Heat inactivation characteristics differed for acidic (A), neutral (N), and basic (B) broccoli peroxidase. At 65 °C, A was the most heat stable followed by N and B.
The activation energies for denaturation were, and kJ/mol for A, N, and B, respectively. Reactivation of N occurred rapidly, within 10 min after the heated enzyme was cooled and incubated at room temperature.
The. However, Concanavalin A failed to show any effect on the enzyme solubilized in C12E8 at a low ionic strength. With solubilized Mg2+ -ATPase from hepatoma plasma membranes, Concanavalin A could not prevent the inactivation of the enzyme during incubation at degrees C.
PMID: [PubMed - indexed for MEDLINE] MeSH Terms. Disinfection of bacterial viruses MS2, Q β, and φ X in water was successfully carried out using deep ultraviolet light-emitting diodes (DUV-LEDs) operated at nm and nm.
It was shown that the nm DUV-LEDs are much more suitable than the nm DUV-LEDs for the disinfection of water because, even though the disinfection efficiency of the former DUV-LEDs is only .Predicted Inactivation of Viruses of Relevance to Biodefense by Solar Radiation C.
Lytle, Jose-Luis Sagripanti Journal of Virology Characterization of Triatoma virus, a picorna-like virus isolated from the triatomine bug Triatoma infestans.The inactivation rates of the small RNA viruses, poliovirus 1 and Coxsackievirus B4, by LP UV were very rapid and reached ~4 log 10 and >5 respectively, within a UV dose of 30 mJ/cm 2.
In contrast, the inactivation of the small RNA bacteriophage, MS2, was much slower and only 2 log 10 inactivation was achieved at a UV dose of 30 mJ/cm 2.